Austria

Zenit G-Sight

HEp-2 CELL SUBSTRATE SLIDES

All our substrate slides locally distributed are compatible with the A. Menarini Diagnostics Zenit series platforms and can be assessed either visually with a fluorescence microscope or automatically with the A. Menarini Diagnostics interpretation system Zenit G Sight.

CHARACTERISTICS

ImmunoConcepts Fluorescent HEp-2 ANA

ImmunoConcepts Fluorescent HEp-2000 ANA

 
  • Abundant easy-to-read mitotic cells
  • Clear pattern identification
  • Ready-to-use pattern controls and conjugates
  • Positive pattern controls for: homogeneous, centromere, speckled, nucleolar
  • Titrable control referenced to the WHO standard 66/233 for ANA
  • Abundant easy-to-read mitotic cells
  • Clear pattern identification
  • Ready-to-use pattern controls and conjugates
  • Enhanced detection of SS-A/Ro autoantibodies
  • Positive pattern controls for: homogeneous, centromere, SS-A/Ro, speckled, nucleolar
  • Titrable control referenced to the WHO standard 66/233 for ANA
  • Includes a positive SS-RA/Ro control as recommended in NCCLS document 1/LA2-A, i/Quality Assurance for the indirect Immunofluorescence Test for autoantibodies to Nuclear Antigens. Approved guideline IA
 

ORDERING INFORMATION

Our products are conveniently available in all parts of Europe through a network of local A. Menarini Diagnostics branches. Please contact your nearest branch for additional information and order placing.

DIAGNOSTIC SIGNIFIANCE OF ANTI-NUCLEAR ANTIBODIES

IIF staining pattern

Nature of antigen

Associated disease

     

Homogeneous / Peripheral

Homogeneous/Peripheral

Deoxyribonucleoprotein
DNA

SLE with renal involvement
SLE

Nucleolar

Nucleolar

4S-6S RNA probably U3 RNA

Scleroderma

Speckled

Speckled

RNP
Sm
SS-A/SS-B
Scl-70

SLE or Mixed Connective Tissue Disease
SLE
SLE or Sjögren’s
Scleroderma

Centromere

Centromere

Inner and outer plates of kinetochore

CREST Syndrome

Cytoplasmic / Mitochondrial

Cytoplasmic/Mitochondrial

70 kd mitochondrial protein

primary biliary cirrhosis

Anti-nuclear antibody (ANA) detection: Zenit G Sight vs visual assessment

ANAs were determined by IIF using HEp-2 cells as a substrate. Tests were conducted blinded on 120 sera from patients with Connective Tissue Diseases or other autoimmune diseases and healthy subjects. Results were evaluated by three independent expert physician examiners and compared with evaluation by the Zenit G Sight automated interpretation system. Excellent agreement (100%) was obtained for the differentiation of positive and negative samples. Discrepancies were observed only in sera identified by Zenit G Sight system as “doubtful negative”: 8% of these were reported “positive speckled” by examiners.

ZENIT G-Sight Manufacturer: Visia Imaging S.r.l.

Manufacturer: A. Menarini Diagnostics S.r.l.

Our values

4 values, which have given life to Menarini and shaped its history

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